B Pilot CX750
Automated CHROM & TFF Dual-Function System
B Pilot CX750 system is a pilot-scale purification platform that integrates both chromatography (CHR) and ultrafiltration (TFF). With an optimized flow-path architecture and upgraded monitoring modules based on previous B Pilot system, CX750 enables easy switching between chromatography and ultrafiltration on a single unit—with no hardware modification or tubing reconnection. Featuring a highly integrated system design, wide process compatibility, and full GMP-compliant capabilities, CX750 provides a reliable and efficient platform for process scale-up and process validation for antibodies, vaccines, recombinant proteins, other biologics, and for CGT manufacturing.
Dual-Function
Wide Compatibility
GMP-Compliant
Flexible and Scalable
Reduced Footprint and Less Capital
A single system supports both CHR and TFF processes. No need to disassemble or reconnect tubing.
The range of flow rates and membrane areas meets different needs from process development to pilot-scale production.
Compatible with most chromatography column and TFF cassette; suitable for purification of mAbs, vaccines, recombinant proteins, and gene therapy products.
Chromatography and ultrafiltration functions are integrated into one platform, reducing cleanroom footprint and capital expenditure.
The fully automated platform acquires pilot-scale process parameters for key steps, ensuring seamless process transfer.
The system architecture is adapted to future process upgrades and scale-up requirements.
Material compliance: All product-contact components meet USP Class VI requirements, ensuring biocompatibility and cleanability.
Data integrity: Supports electronic signatures, audit trails, and complete data records, in full compliance with 21 CFR Part 11.
Cleanability: Supports CIP, enabling thorough cleaning and safe switching between batches and products.
Basic Specifications of the CX750 System
| Name | Specification | Name | Specification |
| System Dimensions L×W×H | 708×700×820 mm | Equipment Weight | 90 Kilograms (Kg) |
| Pipeline Specification | Polytetrafluoroethylene (PTFE) material; Outer Diameter (OD): 3/16" (4.8mm), Inner Diameter (ID): 1/8" (3.2mm) | Maximum Working Pressure | 6 bar |
| Operating Temperature | 2~40℃ | Operating Humidity | 5%~95% (non-condensing) |
| Power Supply Requirement | 100~240 Volts (V), 50~60 Hertz (Hz) | System Power | 1100 Watts (W) |
| Cabinet Material | Aluminum alloy with powder coating | System Dimensions | Internal pipeline flow path volume: 35ml (dual-pump configuration); Bubble trap volume: 70mL; Mixer volume: 5ml |
| Software | Standard configuration: CBS 1.2.0; Optional: DCS (Distributed Control System) | Control System | Controller: Siemens PLC150; Workstation: Standard desktop PC; Optional: Wireless tablet |
Chromatography System Specifications
| Name | Specification | Name | Specification |
| System Pump | Dual-pump gradient system: A/B diaphragm pumps; Single pupm: 30~750 mL/min; Pressure resistance: 0~6 bar | Ultrasonic Flow Meter | Accuracy±2% |
| UV Detector | Wavelength: 200~400 nm; Detection: -5~5 AU; Linear accuracy: ±2% (0~2AU); Light path: 2 mm; Wavelength: ±2 nm; Optional LED service life>8000 hrs | Conductivity Detector | Measurement: 0.02~300 mS/cm; Accuracy: <±3%>20 μS/cm; Integrated temp. sensor; Temp.: 0~99℃; Accuracy: ±2℃ (2~50℃) |
| pH Detector | Measurement range: 0~14; Accuracy: ±0.2 (2~12 pH) | Pressure Detector | Measurement range: 0~6 bar |
| Column Position Valve | Optional: Single-column / Dual-column configuration | Inlet Ports | Maximum inlets: 12 (A1~A6, B1~B6) |
| Outlet Ports | Maximum number of outlets: 6 (P1~P6) | Flow rate accuracy | ±2% or ±5 mL, whichever is greater |
Ultrafiltration System Specifications
| Name | Specification | Name | Specification |
| Recirculation Flow Rate | 30~750ml/min | Compatible Membrane Area | 0.01~0.2m2 |
| TMP Adjustment Mode | Automatic Control | Cross Flow (CF) Adjustment Mode | Automatic Control |
| Pressure Detector | Range: 0~6 bar; Accuracy: ±0.2 bar | UV Detector | Wavelength range: 200~400 nm; 4-wavelength detection; Light path: 2 mm; Measurement range: 0~2 AU; Linear accuracy: ±2% |
| Conductivity Sensor | Measurement range: 0.02 ~ 300 mS/cm; Accuracy: ±3% or ±20μS/cm | pH Detector | Measurement range: 2~12 pH; Accuracy: ±0.2 |
Software Platform
Compliant design: meets 21 CFR Part 11 requirements with electronic records, electronic signatures, access control, and password management.
Flexible recipe editing: allows configuration of flow rate, valve switching, gradient modes (CV, L, or min), and other operational parameters based on process requirements.
Audit trail: fully records all operations with support for generating PDF reports.
Data backup and recovery: supports automatic and manual backup to ensure data integrity and traceability.
System log recording: provides comprehensive operation logs for process and system diagnosis.
Alarm and warning management: supports alarm configuration and recovery logic to improve operational safety and system reliability.
Database-based data management: All data is stored in a database to ensure stability and searchability.
